Биология ва тиббиёт муаммолари 2024 №2 (152)


Subject of the article

LABORATORY INDICATORS IN RATS EXPOSED TO ISOLATED AND COMBINED EXPOSURE TO LEAD AND CHROME (276-281)

Authors

Kim Tat`yana Aleksandrovna, Mavlyanova Zilola Farkhadovna

Institution

1 - Asfendiyarov Kazakh National Medical University, Republic of Kazakhstan, Almaty; 2 - Samarkand State Medical University, Republic of Uzbekistan, Samarkand

Abstract

Knowledge of the nature of disorders of the endocrine apparatus of the pancreas as a result of exposure to heavy metals and the development of diabetes mellitus under these conditions will contribute to a deeper understanding of the pathogenesis of this disease and, consequently, the creation of rational methods of prevention. In this connection, the article presents some aspects of the pathogenesis of the damaging effects of lead and chromium compounds on the en-docrine function of the pancreas of rats. To conduct the experiment, depending on the substance that caused the intoxication, 4 groups of animals were formed: 1 group – “lead” (n=33); Group 2 – “chrome” (n=40); Group 3 – “lead+chromium” (n=108); Group 4 - “ intact ” (n=47). It has been shown that combined exposure to metals leads to more pronounced activation of free radical oxidation processes, as a result of which excess lipid peroxidation is induced, which in turn leads to greater damage to pancreatic β- cells and the development of hypoinsulinemia in these animals, compared with isolated exposure metals, where hypoinsulinemia was not observed. The presence of mortality and a greater decrease in body weight in the “lead + chromium” group, in contrast to the comparison groups, also indicate a more pronounced toxic effect of the combination of metals on the body compared to their isolated effect.

Key words

lead, chromium, pancreas, rats, lipid peroxidation, antioxidant system.

Literature

1. Frolov V.A. Environmental pathophysiology // Pathological physiology and experimental therapy. – 2020. - No. 4. – P. 2-5. 2. The influence of lead on the development of oxidative stress / I. M. Trakhtenberg [et al.] // Toksikol. Vestn. 2019. No. 3. P. 22–25 3. Ercal, N. Toxic metals and oxidative stress part I: mechanisms involved in metal-induced oxidative damage / N. Ercal , H. Gurer-Orhan, N. Aykin- Burns // Curr Top Med Chem. 2018Vol. 1, No. 6. R. 529−539. 4. Ametov A.S. Prospects for the development of diabetology // Therapeutic archive. – 2012. - No. 10. – P.5-9. 5. Zhetkovsky S.S., Petrova L.V., Ametov A.S. Current state of problems in the prevention and treatment of diabetes mellitus // Therapeutic archive. – 2016. - No. 10. – P. 46-50. 6. Okshina L.N. On the issue of the state of the endocrine function of the pancreas in lead plant workers // Research Institute of Regional Pathology of the Ministry of Health of the Kazakh SSR, 1982. - T. 47. - P. 10-11. 7. Zeltser M.E. Study of the epidemiology of diabetes mellitus in Kazakhstan. Materials 1 Conference of Diabetologists of Central Asia. Almaty, 1994. 8. Udartseva T.P. The influence of diesel exhaust gases on the endocrine function of the pancreas. Materials 1 Conference of Diabetologists of Central Asia. Almaty, 1994. 9. Dragonay K.V. On the early detection of diabetes mellitus and hypothyroidism in metallurgical industry workers. Abstract of the dissertation. Chelyabinsk, 1975. 10. Korolyuk M.A., Ivanova L.I., Mayorova I.G. Method for determining catalase activity // Lab. case. - 1988. - No. 1. – P. 16-19. 11. Lvovskaya E.I., Volegorodsky I.A., Shemyakov S.E. Spectrophotometric determination of end products of lipid peroxidation // Questions of honey. x imii. - 1991. - T. 37, No. 4. - P.334336 12. Rakitsky V.N., Yudina T.V. Methodological approaches to assessing indicators of oxidative stress under the influence of anthropogenic environmental factors // Hygiene and Sanitation. – 2017. - No. 5. – pp. 28-30. 13. Камалова Ё. Особенности физической реабилитации остеохондроза поясничного отдела позвоночника //InterConf. – 2020. 14. Umirova S. M., Mavlyanova Z. F., Sabirova Sh B. Rehabilitation measures for protrusion of intervertebral discs of the lumbar spine in athletes engaged in various types of martial arts //Achievements of science and education. – 2019. – Т. 12. – №. 53. – С. 68-71. 15. Абдуллаев Р. Б. Особенности клинического течения, распространенности и новые подходы к лечению язвенной болезни желудка и двенадцатиперстной кишки в Южном Приаралье //Автореф. дисс.... докт. мед. наук. Ташкент. – 2002. – С. 15-23. 16. Абдуллаев Р. Б., Якубова А. Б. Хроник гепатит (экологик ноқулай хисобланган Жанубий Орол бўйи худудида касалликнинг ўзига хос кечиши ва уни даволаш) //Хоразм‖ нашриѐти Урганч. – 2008. 17. Абдуллаев Р. Б., Ходжаев Ш. А., Рузметова М. С. Распространенность экстрагенительных заболеваний у женщин фертильного возраста, проживающих в условиях экологического необлагополучия //Мед. журн. Узбекистана. – 2000. – Т. 4. – С. 65-67. 18. Худойкулова Ф. В. и др. the structure, age features, and functions of hormones. pedagog, 1 (5), 681-688. – 2023. 19. Sharafova I. A. et al. Kinesiotaping: New Look at the Rehabilitation of Facial Nerve Neuropathy in Children //Annals of the Romanian Society for Cell Biology. – 2021. – С. 4763-4768. 20. Баратова С., Мавлянова З., Шарафова И. Изучение темперамента спортсменок, занимающихся таэквондо //Журнал вестник врача. – 2019. – Т. 1. – №. 2. – С. 30-32. 21. Anatolevna, Kim Olga, Abdusalomova Maftuna Akbarovna, and Makhmudov Sardor Mamasharifovich. "Zhalolitdinova Shaxnoza Akbarzhon kizi, & Ibragimova Leyla Ilxomovna. (2022). the influence of risk factors on the development of cerebral strokes in children. open access repository, 8 (04), 179–182." 22. Абдусаломова М. А., Мавлянова З. Ф., Ким О. А. Орқа мия ва умуртқа поғонасининг бўйин қисмининг туғруқ жароҳатлари билан беморларнинг диагностикасида электронейромиографиянинг ўрни //журнал биомедицины и практики. – 2022. – Т. 7. – №. 2.